Fig. 7. ROS production was measured by DCF fluorescence after 6 h of normal atmospheric gas pressure, IH or SH in our model of blood-brain barrier. 1000 µM H₂O₂ was used as a positive control. DCF fluorescence levels were measure after these conditions. Values are presented as mean value ± SEM (n=9, N=3), * p≤0.05, ** p≤0.01, *** p≤0.001 compared to control condition. SH: sustained hypoxia, IH: intermittent hypoxia, control: normal atmospheric gas pressure, ROS: reactive oxygen species, DCF: dichlorodihydrofluorescein.